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one-step

标签:TimeThisrealprotocoldescribesone-step

This protocol describes one-step real time quantitative reverse transcription PCR to quantify relative levels of a particular mRNA sequence between two samples. This technique is also called known commerically as Taqman, qRT-PCR, real-time PCR. This parti

标签:proteinExtractionmethodprotocolone-step

1. One-step extraction method: 在冰上将组织在匀浆缓冲液中(25mM NaH2PO4 pH7.2,1.5mM EDTA,10% Glycerol,10mM Na2MO4,10mM NaF,2μM Aprotinin,5μM Leupeptin,2mM PMSF)剪碎,用高速搅拌器匀浆,而后于10,5000g 超离心一小时。取上清,用Brandford法定蛋白浓度

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