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标签:纯化蛋白分离protein概述Isolationoverview

Isolating a protein may be compared to playing a game of golf. In golf, the player is faced with a series of problems, each unique and yet similar to problems previously encountered. In facing each problem the player must analyse the situation and decide,

标签:blotNorthernHowThisProcedurebriefoverview

This is a brief overview of how a Northern blot (more formally called an RNA blot) is performed and what type of data you can obtain form one. Northern blots allow investigators to determine the molecular weight of an mRNA and to measure relative amounts

标签:RNADNaseTotaloverviewmostTreatmentextractions

Overview Most RNA extractions procedures yield RNA, with minimal or no contamination with DNA, that is suitable for applications such as northern blot, constructions of cDNA libraries or microarray hybridizations. However, applications that rely on specif

标签:RNAuseoverviewAmplifyingReversewith

Overview The use of reverse transcriptases to perform first-strand cDNA synthesis has had a huge impact on the study of biological processes. Finally, an RNA could be converted into DNA, allowing manipulation and cloning. Another important milestone in th

标签:blotmethodHowSouthernThisbriefoverview

This is a brief overview of how a Southern blot (more formally called an DNA blot) is performed and what type of data you can obtain form one. Figure 1. Southern blots allow investigators to determine the molecular weight of a restriction fragment and to

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