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标签:RFLPmethodWordpronouncedriflipwere

RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFL

标签:blottingWesternmethodDepartmentMichiganState

Department of Medicine, Michigan State University, East Lansing, Michigan, USA. hongbao@msu.edu Abstract: Western blotting is a useful and sensitive method to measure the ng/ml to pg/ml ordered materials in the solution, such as serum, urine and culture s

标签:andTissuemethodLabUsingOurwassuccessfulhisprostate

his method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study. Solutions ( TIP: Use ele

标签:cDNAmethodUsingrealabsolute

Joseph A. Whelan, Nick B. Russell, Michael A. Whelan* Onyvax Ltd., St. George’s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK Received 3 February 2003; received in revised form 1 May 2003; accepted 1 May 2003 Abstract Real-time PCR is an e

标签:DNACTABIsolationExtractionmethodtechniqueSchedule

CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) Reagents needed CTAB buffer 2% CTAB 20gm CTAB 20mM EDTA 40ml EDT

标签:RNADNAExtractionmethodFromMaterialprotocolSame

A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also DNA Isolation protocol ) 1) Take one medium sized leaf or half a large leaf (5 to 20 cm^2), weigh and f

标签:PCRdNTPProbeMaterialsmethodmakeRadioactiveProbesHot

Materials Hot Probe dNTP Mix: 25 mM dATP 25 mM dTTP 25 mM dGTP 2.5 mM dCTP Hybridization Solution: 5X SSC 0.5% (w/v) Blocking Reagent 0.1% (w/v) N-lauroylsarcosine, Na-salt 0.02% (w/v) SDS 50% Formamide Blot Wash 1: 2X SSC 0.1% SDS Blot Wash 2: 0.1X SSC 0

标签:methodThiswasReversesubmittedJim

This method was submitted by Jim Hutchins from the University of Mississippi Medical Center. The following is a great RT-PCR protocol developed by Ignacio Rodriguez at the National Eye Institue, NIH. He gets all the credit; if you have problems with it, c

标签:proteinExtractionmethodprotocolone-step

1. One-step extraction method: 在冰上将组织在匀浆缓冲液中(25mM NaH2PO4 pH7.2,1.5mM EDTA,10% Glycerol,10mM Na2MO4,10mM NaF,2μM Aprotinin,5μM Leupeptin,2mM PMSF)剪碎,用高速搅拌器匀浆,而后于10,5000g 超离心一小时。取上清,用Brandford法定蛋白浓度

标签:GeneRT-PCRmethodThisOurwassuccessful

Gene-specific RT-PCR This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study. To

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