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Extraction

标签:RNAandExtractionTotalFromtobacco

RNA extraction buffer: 0.1M NaCl 2% SDS 50mM Tris/HCl (pH9) 10mM EDTA 20mM -mercaptoethanol 1. Grind leaf tissue on liquid N in mortar and pestle. 2. Transfer the ground tissue to a 10ml conical bottom centrifuge tube (don’t let the tissue thaw) that cont

标签:RNA提取方法哈佛HumanExtractionprotocolREAGENTS

REAGENTS Guanadinium Thiocyanate 1.0 M Sodium Citrate pH 7.0 10% Sarcosyl 2-mercaptoethanol water saturated phenol pH 4.0-7.0 Na2EDTAMOPS (free acid) 2.0 M Sodium Acetate pH 4.0 Formaldehyde Formamide 10% SDS 49:1 Chloroform:Isoamyl Alcohol SOLUTIONS filt

标签:DNABruceExtractionsamplesFromadaptedRoePhenol

(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019 broe@ou.edu) Phenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated pheno

标签:DNACTABIsolationExtractionmethodtechniqueSchedule

CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) Reagents needed CTAB buffer 2% CTAB 20gm CTAB 20mM EDTA 40ml EDT

标签:RNADNAExtractionmethodFromMaterialprotocolSame

A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also DNA Isolation protocol ) 1) Take one medium sized leaf or half a large leaf (5 to 20 cm^2), weigh and f

标签:蛋白质提取protein制备andExtraction

蛋白质提取与制备蛋白质种类很多,性质上的差异很大,既或是同类蛋白质,因选用材料不同,使用方法差别也很大,且又处于不同的体系中,因此不可能有一个固定的程序适用各类蛋白质的分离。但多数分离工作中的关键部分基本手段还是共同的,大部分蛋白质均可溶于水、稀盐、稀酸或稀碱溶液中,少数与脂类结合的蛋白质溶于乙醇、丙酮及丁醇等有机溶剂中。因此可采用

标签:andExtractionProteinsFromDigestionAcrylamidePeptides

Sherry Niessen, Ian McLeod and John R. Yates III Department of Cell Biology, The Scripps Research Institute, La Jolla, California. Excerpted From Protein: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams. ABSTRACT Analysi

标签:proteinExtractionmethodprotocolone-step

1. One-step extraction method: 在冰上将组织在匀浆缓冲液中(25mM NaH2PO4 pH7.2,1.5mM EDTA,10% Glycerol,10mM Na2MO4,10mM NaF,2μM Aprotinin,5μM Leupeptin,2mM PMSF)剪碎,用高速搅拌器匀浆,而后于10,5000g 超离心一小时。取上清,用Brandford法定蛋白浓度

标签:RNAMiniExtraction沉淀法氯化Prep

RNA的制备与分析对于了解基因在转录水平上的表达与调控和cDNA的合成都是必须的,RNA的纯度和完整性对于Northern blot,RT-PCR和cDNA文库的构建等分子生物学实验都至关重要。RNA分离的方法很多,其中最关键的因素是尽量减少RNA酶的污染 实验目的: 学习RNA的简易制备过程,通过RNA电泳带评价RNA质量 实验材料: 水稻幼嫩叶片 实验原理: SDS是一种去污剂,可以抑制内源RNA酶的活性,

标签:RNATrizol制备MiniExtractionPrep

RNA的制备与分析对于了解基因在转录水平上的表达与调控和cDNA的合成都是必须的,RNA的纯度和完整性对于Northern blot,RT-PCR和cDNA文库的构建等分子生物学实验都至关重要。RNA分离的方法很多,其中最关键的因素是尽量减少RNA酶的污染 实验原理: Trizol 试剂是由苯酚和硫氰酸胍配制而成的单相的快速抽提总RNA的试剂,在匀浆和裂解过程中,能在破碎细胞、降解细胞其它成分的同时保

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