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标签:andXenopusGuidelinesEggOocyteHarvestinglaevis

Amphibian oocytes are used for studies in molecular biology, embryology and biochemistry.Unfertilized eggs are collected by gently squeezing eggs from females which have been injected with human chorionic gonadotropin (HCG) - 500 IU injected into the dors

标签:染色质标本观察andchromatin

X and Y Chromatin Preparation and Observation 【实验目的】 熟悉X染色质和Y染色质的形态特征及其检查方法 【实验原理】 正常女性核型为46,XX。在间期细胞核中紧贴核膜内缘有一个染色较深的椭圆形小体,即X染色质,这是女性细胞中一条X染色体随机Lyon化失活形成的。这种失活保证了雌雄两性细胞中都只有一条有活性的染色体,使两性X连锁基因产物的量保持在相同水平上。称为X染色体的

标签:andBacterialSDSRECOMBINANTexpressionCultureObtain

1. Obtain a bacterial culture containing a Protein X (Lec-1) expression vector from your TA. There are two types, A and B – one is grown in the absence of arabinose, one has been arabinose induced – it is your job to work out which is which. 2. Take 500ml

标签:andTissuemethodLabUsingOurwassuccessfulhisprostate

his method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study. Solutions ( TIP: Use ele

标签:plasmidandPurposePurificationShuttle

1.0 Purpose 1.1 This protocol may be used for the linearization and purification of the shuttle plasmid. 2.0 Materials 2.1 Equipment DNA Gel Electrophoresis Apparatus Heating block set at 65 C Centrifuge -20 C Freezer UV Spec 2.2 Supplies Restriction Enzy

标签:CellsandPreparingUsingBacterial

Evening before: 1. Set up overnight culture of bacteria (e.g. DH5-alpha) in 100 ml LMM. 2. Autoclave several flasks of 2X YT broth (1 litre per flask). Aim to process at least 2 flasks. Also autoclave 3 litres of 10% glycerol water (381 g of glycerol diss

标签:AgarandAntibioticStockPreparationSolutionsplates

Antibiotic Stock Solutions Ampicillin Beta-lactam-antibiotics are not very stable when dissolved. Slow but steady degradation happens even when frozen to -20°C. Therefore commercial beta-lactam-antibiotics are sold as dried powder and reconstituted just b

标签:GTPandMakingSEEDSMicrotubulePrechillglycerolMgCl2

Prechill glycerol, MgCl2, and GTP on ice. Rapidly thaw frozen tubulin, then immediately place on ice. Unpolymerized tubulin is labile! 200 L aliquot of tubulin (cycled)--in BRB80, stored in -70℃ 100 L glycerol 10 L 0.1 M MgCl2 3 L 0.1 M GTP (pH adjusted t

标签:电泳全自动andKits解答疑问AnalysisFAQsChips

Analysis Kits (Chips and Reagents) Experion Automated Electrophoresis — Sample Types What types of samples can the Experion separate and analyze? The Experion automated electrophoresis system separates and analyzes protein and RNA (either mRNA or total RN

标签:andRestrictionRapidBisulfitefollowed

Bisulfite-PCR followed by restriction is a rapid and semi-quantitative method of analyzing DNA methylation. The PCR products are also suitable for either direct sequencing or cloning and sequencing. The most important step here is primer selection. Genera

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